EU network of mesocosms facilities for research on marine and freshwater ecosystems open for global collaboration.

AU Lake Mesocosm Warming Experiment (LMWE)

Climate change-Long term experiment following the response of an entire lake ecosystem changes in temperature and nutrient loading. Project lead: Erik Jeppesen. 01/01-31/12 2018

The Lake Warming Mesocosm Experiment (LMWE) has been running an experiment with 2 nutrient levels crossed with three temperatures uninterrupted since August 2003. In June 2018 we plan to continue with high TP loading in the high nutrient mesocosms, but will drop N loading to the level in the low nutrient mesocosms (which only are receiving input with ground water). We will then follow the response of the entire lake ecosystems (structure and processes) the rest of 2018 and welcome researchers and students from abroad to contribute, ideally by measuring before and after the event. The retention times in the mesocosms are ca. 2.5 month.

All AQUACOSM approved TA users are welcome to participate in the long-term experiment from beginning of 2018, preferably before and after June 2018. Expertise on measuring nitrogen cycling and also effects on GHG is of specific interest. In addition we have stored samples of water, phytoplankton and zooplankton (phyto, zoo not counted) from the beginning of the experiment and also welcome scientist and students to contribute to the analyses of those. Studies in the untouched low nutrients mesocosms are also welcome.

At least 300 person-days will be allocated to external users through AQUACOSM Transnational Access provision in 2018. Access is offered to a minimum of 6 persons for 50 days each year.

The number of projects is flexible.

AU Lake Mesocosm Warming Experiment (LMWE)
Legal name of organisation
Aarhus University (AU)
Organisation address

Aarhus University (AU)
Nordre Ringgade 1
8000 Aarhus C

Primary contact information (PI)

Prof. Erik Jeppesen

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Years of Mesocosm Experiments
Description of Facility

outdoor – pelagic/benthic – freshwater

24 cylindrical outdoor mesocosms, each 2.8 m3 in volume

Controlled Parameters

Nutrients, temperature

Research Topics

A unique long-term flow-through mesocosm facility with 11 years so far the world’s longest running mesocosm experiment addressing climate-change effects on lakes under contrasting nutrient levels and water clarity, population- and seasonal dynamics of underwater plants, plankton, production, respiration, stable isotopes, bacteria, fluxes of nutrients

Facility location(s)
Primary interests
Specialist areas
Source of Information
Photos of experiments/installations
Schema of mesocosm tank
Mesocosm tanks in Silkeborg, DK


Infrastructure (short name)
AU Lake Mesocosm Warming Experiment (LMWE)
Installation (short name)

Silkeborg, Denmark

Coordinates / (routes, areas if non-static)
Water / environmental conditions
Depths / bottom / surface

The AU Lake Mesocosm Warming Experiment (LMWE) is a unique long- term (with 12 years so far the world’s longest running) flow-through mesocosm experiment addressing climate-change effects on lakes under contrasting nutrient levels and water clarity. Twenty-four cylindrical outdoor mesocosms are used, each with a capacity of 2.8 m3 (Fig. 6.13.1). The mesocosms receive groundwater 6 times per day, resulting in a theoretical water retention time of 2.5 months. Water in the mesocosms is continuously mixed by paddles and heated by electrical elements. Three temperature regimes are run: ambient temperature and two elevated temperatures according to IPPC climate scenarios A2 and A2 + 50%, down-scaled to local 25 25 km grid cells. The modelled temperature difference for the A2 scenario is generally higher in August to January (max. 4.4 °C in September) than during the rest of the year (min. 2.5 °C in June). Individual mesocosms of each temperature regime are run at either low or high nutrient concentrations (4 replicates per treatment). The latter are obtained by weekly dosing of N and P.

Located 15 km from AU Silkeborg, the facility is equipped with a small lab building to facilitate accessory small-scale studies and analyses. A fully equipped lab is available at the AU freshwater central lab in Silkeborg. The mesocosm facility is on the road map of infrastructures as part of a Danish AnaEE project and it is also included in the European AnaEE list of experimental facilities for ESFRI.

Fig. 6.13.1. Aerial view (left) and schematic (right) of the Lake Mesocosm Warming Experiment (LMWE) in Denmark.

Instruments / sensors / equipment

Permanently installed probes in all mesocosms for oxygen and pH measurements, AU freshwater lab in Silkeborg (15 km)

Modality of access

A total of 900 person-days will be allocated to external users through AQUACOSM Transnational Access provision in years 2-4. Access is offered to a minimum of 6 persons for 50 days each year.

Unit of access
What is available and when
What service and support facilities are available

Services currently offered by the infrastructure: The LMWE provides a unique opportunity to test for long- term warming impacts on trophic dynamics, nutrient balances, ecosystem metabolism, greenhouse gas exchange, adaptation and microevolution that are not possible at other locations in the world. Users have access to the laboratories, instrumentation, etc. at both the mesocosm facility and the AU freshwater lab in Silkeborg. Users of LMWE meet a highly international scientific community with many guests from both Europe and overseas. This includes numerous students and postdocs from Europe, Israel, China, Argentina and Turkey. Close cooperation with local scientists is encouraged to maximize outcomes.

The facility was used in the FP7 REFRESH project and is currently included in the FP7 project MARS (, running from 2014-2018, to assess effects of a heat-wave on shallow lakes. Additional projects are the European JPI Water project PROGNOS (2016-2019) assessing in-lake responses to change using near real-time models, and a Danish lake restoration project (CLEAR from 2012-2017; a Villum Kann Rasmussen Center of Excellence on lake restoration) involving a 15N spiking experiment.

Support offered under AQUACOSM: All visitors will have access to the laboratories and instrumentation available at the facility. Beyond access to the LMWE, all users will receive scientific support for planning activities before and after arrival (typically initiated several months in advance) and hands-on training by the local scientific team. The visiting scientists will meet 1) an internationally leading team of aquatic scientists, headed by one of the most cited aquatic ecologists in the world and 2) a stimulating international environment with numerous scientists, students and postdocs from around the globe. The visitors will further benefit from competent technical and administrative staff associated with the facility, who can provide technical advice and assist with the purchase of consumables, lodging and other technical and administrative issues.

Special rules