EU network of mesocosms facilities for research on marine and freshwater ecosystems open for global collaboration.


The CRETACOSMOS mesocosm facility plans to open Transnational Access for two experiments in 2019.

Timing of the experiments (planned):

Experiment 1) mid May to mid June 2019

Experiment 2) mid September to mid October 2019

More information about the topic, objectives and detailed description of the experiments will follow in the next weeks.

Contact: Paraskevi Pitta

We plan to open our facility for AQUACOSM TA either in May/June or in September/October 2018. We have already contacted some colleagues and have preliminary plans about a couple of experiments. We intend to set up a group of people to come to our facility and according to the interest that will be expressed for our facility.

At least 300 person-days will be offered to external users supported through the AQUACOSM Transnational Access provision in years 2018, that is 6-8 persons for 25-30 days. Projects of external users will be integrated in the general schedule of the facility.

Legal name of organisation
Hellenic Centre for Marine Research (HCMR)
Organisation address

Institute of Oceanography
Ex American Base Gournes
71003 Heraklion, Crete

Primary contact information (PI)

Dr. Paraskevi Pitta

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Years of Mesocosm Experiments
2009 to present
Description of Facility

outdoor – pelagic/benthic – marin

12 polyethylene mesocosm-bags up to 5 m3 (1.32 m diameter) incubated in two large volume concrete tanks (one with volume 150 m³ and 3 m depth and a second one with volume 350 m³ and 5 m depth)

9 benthocosms combining water column (1.5 m3, 4 m deep, 0.64 m diameter) and sediment (85 L) in the same polyethylene mesocosm-bag

The smaller (150 m³) tank is equipped by a sophisticated, fully-automated heating/cooling water system with electric valves, resistances and temperature sensors that allow water heating/cooling and temperature control at ±0.5 ºC of targeted temperature

Controlled Parameters

Temperature, light, nutrients

Research Topics

Response of the ultra-oligotrophic food web of the Eastern Mediterranean to perturbations, climate change scenarios (simultaneous effect of warming & acidification), impact of aerosol input, effect of silver nanoparticles, benthic-pelagic coupling at benthocosms, hypoxia

Facility location(s)
Primary interests
Specialist areas
Source of Information
Photos of experiments/installations
Cretacosmos facility during the LightDynaMix project (Photo: Stella A Berger)
Two basins of the Cretacosmos mesocosm facility (Photo: Vivi Pitta)
Cretacosmos mesocosm facility (Photo: Vivi Pitta)


Infrastructure (short name)
Installation (short name)

Ex American Base, Crete, Greece, 25 km east of Heraklion

Coordinates / (routes, areas if non-static)
Water / environmental conditions
Depths / bottom / surface

CRETACOSMOS is part of the Hellenic Centre for Marine Research (HCMR) in Crete. It is the only place in Europe where truly oligotrophic conditions can be studied in a well- controlled mesocosm facility. The CRETACOSMOS facility includes 12 pelagic mesocosms (3 m ) and 9 benthocosms, which are incubated in two large temperature-controlled concrete tanks (150 m volume and 3 m depth, and 350 m volume and 5 m depth, respectively; Fig. 6.4.1). The pelagic mesocosms are low- density polyethylene bags of 1.32 m diameter, enclosing 3 m3 of water. The benthocosms are low-density polyethylene bags 0.64 m in diameter and 4.5 m high. They hold 1.5 m3 of water and a container with 85 L of sediment at the bottom. The smaller (150 m ) tank is equipped with a fully automated heating/cooling system that allows temperature manipulation and control within ±0.5 oC of the target temperature. Water of the Eastern Mediterranean is collected off Heraklion aboard the R/V Philia, a 26 m long research vessel, and transported to the facility by lorry to fill the mesocosms. Given the oligotrophic nature of the water, special care is taken to avoid contamination. A lid protects the mesocosms from aerosol deposition. Water mixing is achieved by gentle bubbling into a vertical PVC tube, creating an airlift from just above the mesocosm bottom to just under the surface. The possibility to heat water and control its temperature allows running climate-change experiments since 2013. Similarly, the benthocosms, built in 2014 and containing a relatively large sediment volume, open the possibility to run experiments on benthic-pelagic coupling. In addition, the concrete tanks can be used to test large instruments.

The mesocosm facility is complemented by modern laboratories: a chemical lab to analyse dissolved and particulate nutrients; a radio-isotope lab (14C, 3H, 33P) to measure primary and bacterial production and phosphorus uptake; a culture lab equipped with an autoclave, laminar-flow cabinet, incubator, etc.; an automotive lab obtained in 2015; several general-purpose labs and a constant-temperature room. Other equipment includes a -80°C freezer and a flow cytometer (FACSCalibur) to count viruses, bacteria and cyanobacteria as well as pico- and nanoflagellates. Inverted and epifluorescence microscopes with automated image analysis systems are available to identify and count nano- and micro-plankton. The facility is also supported by a zodiac.

Fig. 6.4.1. Panoramic view of the CRETACOSMOS facility (left), sampling of the mesocosms (middle), view of the benthocosms (right). Photos: P. Pitta.

Seven large-scale experiments have used the CRETACOSMOS facility since 2009: Nutritunnel (2009: Norwegian Research Council), MESOAQUA (2010 and 2012: EU-FP7), MedSeA (2013: EU-FP7), ADAMANT (2014: EU Social Fund plus national funding); HYPOXIA and AQUANANO (2014 and 2015, respectively: both EU and national funding). Additionally, an enclosure facility moored offshore; the Lagrangian Mesocosm Platform (LAMP) operated by Partner 10 (CNRS-MARBEC), was successfully tested in 2011. Over 90 scientists from 22 countries in Europe, the Americas and Asia participated in these experiments, addressing various aspects of global environmental change. For example, the experiment in 2013 was the first to assess the combined effects of seawater warming and acidification on the pelagic food web of the oligotrophic Eastern Mediterranean, and the benthocosms were first used during a two-month experiment addressing the effect of eutrophication on benthic-pelagic coupling.

Instruments / sensors / equipment

chemical lab, radio-isotope lab (14C, 33P, 3H), flow cytometry lab, microscopy lab (inverted and epifluorescence microscopes, image analysis system), culture/live laboratory, constant temperature room and several general use labs, 26m long research vessel PHILIA, zodiac

Modality of access

A total of 688 person-days will be offered to external users supported through the AQUACOSM Transnational Access provision in years 2-4, a minimum of 6 persons for 30 days each year. Projects of external users will be integrated in the general schedule of the facility.

Unit of access
What is available and when
What service and support facilities are available

Services currently offered by the infrastructure: Users of CRETACOSMOS have access to the full range of mesocosms, laboratories and instrumentation described above. Visiting scientists supported by AQUACOSM are encouraged to cooperate with local scientists to benefit from specific expertise on the local environmental conditions.

Support offered under AQUACOSM: The laboratories, research vessels and equipment described above will be at the disposal of the users of CRETACOSMOS under AQUACOSM. To maximize scientific advancements and output of the TA activity, users will receive support in terms of planning of activities both before and after arrival. Hands-on training by the local team of academic and technical staff. IT support, a library with on-line access to all major marine science journals will also be provided. Building on dedicated administrative staff and ample experience gained since 2009, an efficient administration mechanism is in place. The HCMR research park in Heraklion (Thalassocosmos) where 31 scientists of 3 institutes work on oceanography, aquaculture, fisheries, marine biology and genetics offers a stimulating broader scientific environment. Visitors under AQUACOSM are encouraged to get involved in the academic discourse in both formal and informal ways.


no dormitories, accomodation in hotels

Special rules