EU network of mesocosms facilities for research on marine and freshwater ecosystems open for global collaboration.

Limnotrons

The Limnotrons at NIOO-KNAW will open for external AQUACOSM TA applicants from March 2021 to July 2021 to perform their own research ideas. Pease apply for TA now!

Contact: Lisette de Senerpont Domis (Please login or register to view contact information.)

The facility will be open for participation of external users approved by AQUACOSM TA as of January 1, 2020 to the end of October 2020 to perform their own research ideas.

Can extreme climatic events affect the robustness of aquatic ecosystem restoration? Project lead: Lisette de Senerpont Domis & Cleo Stratmann. 01 April-31 July 2019. (Description of the planned experiment and scientific background, see below).

Timing of the Limnotron experiment: The large-scale mesocosm experiment is planned for four months. This will include experiment preparation, conduction and after-work, where all participants are expected to participate as an integral part of the application period.

We encourage a wide range of Transnational Access (TA) users to apply: You may come from public authorities, technological partners, and research or teaching institutions. We especially welcome microbiologists, molecular biologists, biogeochemists, and aquatic ecologists in general to participate in our extreme event experiment, and to come up with additional experiments which can be run adjacent to the extreme event experiment.

A total of at ca. 300 person days will be allocated to external users through Transnational Access provided under AQUACOSM for 2019. It is anticipated that AQUACOSM will support stays of at least 5 persons for ca. 50 days, or potentially other combinations.

If you are interested, please contact us (l.desenerpontdomis@nioo.knaw.nl) well before the application deadline, so we can advice you about possible options.

Introduction to the project: Extreme climatic events (ECEs), or episodic weather events that are increasing in frequency and intensity as the climate shifts, often have disproportionately longlasting effects on the functions of lakes and reservoirs. As these ecosystems play an integral role within the environmental and anthropogenic community, it is pertinent to rehabilitate degraded systems to a state where biophysical functions are resilient to the effects of future ECEs. Healthy, oligotrophic freshwaters are thought to be more resilient to the impacts of ECEs than meso- or eutrophic freshwaters. In the proposed project we will investigate to what extent re-oligotrophicated systems compare to eutrophic systems when affecteded by ECEs.

Description of the Limnotron experiment: In a four-month experiment, we will investigate whether rehabilated systems are more resilient to extreme climatic events than non-rehabilated systems (Fig. 1). We will inoculate the mesocosms with sediment and water from a well-studied eutrophic water body.  To four of the limnotrons we will aply phosphorus sorbents to geoengingeer our initial rehabilated conditions. Four limnotrons will remain in their initial eutrophic conditions. During the course of our experiment we will then apply to both treatments (eutropic vs. re-oligotrophied) extreme precipation events, as well as extreme heatwaves. We will use long-term meteorological data as a starting point for designing the exact nature of our climate event scenarios.

Figure 1: Set-up of Extreme Climatic Event experiment.

Measurements:  During the experiment, we will monitor plankton dynamics, microbial dynamics and epipelon dynamics.  These groups will be monitored in terms of biomass, but also elemental composition, and potential toxin  (cyanobacteria) production. In addition, we will assess ecosystem processes, including primary production, decomposition, sediment biogeochemistry and greenhouse gas emission. All limnotrons will be monitored for standard limnological parameters, including measures light (PAR), temperature, pH, conductivity, oxygen, redox potential, turbidity, total Chl a and other algal pigments. We are open to discussing measuring other variables of interest with the TA applicants.

Mantel – Are rehabilated systems more resilient to extreme climatic events than non-rehabilated systems. Project lead: Lisette De Senerpont Domis. 01/04-31/07 2018

We are planning a 4-month experiment for April-July 2018 within the Marie-Curie-ITN MANTEL program. We will investigate whether rehabilated systems are more resilient to extreme climatic events than non-rehabilated systems. The extreme climatic events we will mimic are extreme precipitation events, and extreme heatwaves. We will use phosphorus sorbents to geo-engineer our initial eutrophic starting conditions.

The facility will also be open for participation of external users approved by AQUACOSM TA as of January 1, 2018 to the end of 2018 to perform their own research ideas. We also welcome collaboration outside the Transnational Access provision.

We welcome microbiologists, molecular biologists, biogeochemists, and aquatic ecologists in general to participate in our extreme event experiment, and to come up with additional experiments. The facility will be open for participartion of external users as of April 1, 2018.

At least 300 person days will be allocated to external users in 2018 of AQUACOSM Transnational Access provision. Access will be offered to a minimum of 10 persons for 30 days or other constellations.

Legal name of organisation (short name)
Netherlands Institute for Ecology (NIOO)
Country
The Netherlands
Continent
Europe
Organisation address

Netherlands Institute for Ecology (NIOO)
Droevendaalsesteeg 10
6708 PB Wageningen

Infrastructure (short name)
Limnotrons
Infrastructure address

Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands

Coordinates / (routes, areas if non-static)
Facility location(s)
Description of the Infrastructure

Description of the infrastructure: The Limnotrons are 9 stainless steel indoor mesocosms with a high level of control. The dimensions are: 0.97 m in diameter, depth between 1.32 m (side) and 1.37 m (centre), volume of 922 L. The vessels can be closed with a removable PMMA flange lid. In addition to the possibility to sample vertically with specifically designed integrated water samples, sampling can also be done by using the sampling ports positioned on three depths. Wall growth is prevented by an impeller made of PMMA, with a stainless steel axis and a silicon rubber strip between the outside and the wall. To create different types of mixing more tailored to growth of cyanobacterial scums, oscillating grids have been designed. Originally designed to study pelagic communities, the Limnotrons have been adapted to allow for a benthic community as well. Each Limnotron can have a sediment layer of 10-15 cm, and with the recently purchased high intensity lights, a macrophyte community can be established in these mesocosms. A specific feature of the Limnotrons is its thermostatically regulated microprocessor-controlled temperature regulation system which enables thermal stratification and allows for running complicated temperature scenarios, including episodic events. Volume differences due to temperature settings, evaporation losses and sampling, are compensated by a (refillable) expansion vessel. CO2 levels can be manipulated by providing different CO2/air mixtures with a WITT KM60-2ME gas mixer. Services currently offered by the infrastructure: Individual external users and user groups will have access to the full range of facilities at the NIOO-KNAW. These include: fluorescence microscopes; autoanalyzer for dissolved nutrients, multichannel probes for simultaneous measurements of oxygen, light, pH, conductivity and temperature, PhytoPAM, fluorometer; Coulter particle counter, Flow cytometers for phyto- and bacterioplankton analyses, FlowCAM for analysis of large phyto- and zooplankton, Elemental analyser (particulate C and N contents), Isotope-ratio mass spectrometry (relative abundance of stable isotopes), ICP-MS (quantify trace elements), LC-MS/MS (small organic chemicals, cyanotoxins), modern molecular-biology lab. The facilities are frequently used for experiments by teams of international researchers from different disciplines including ecologists, microbiologists, biogeochemists, and evolutionary biologists. In 2019, an international multidisciplinary team working on climate change and carbon cycling is hosted. This team consist of both internal and external users, assisted by a team of highly skilled technicians at NIOO- KNAW. The laboratory’s broad range of state-of-the-art analytical equipment allows users to tackle innovative, multi- facetted questions, resulting in publications in leading journals. Users will be embedded in an internationally renowned aquatic ecology group providing ample opportunities for networking and large potential for fruitful scientific exchange. The NIOO-KNAW as a whole (~200 employees) provides an international working community, hosting several world- leading scientists.

Limnotrons (l) and scientists at work (r) in the Netherlands. Photo: NIOO-KNAW.
Primary contact information (PI)
Prof. Ellen van Donk
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Lisette de Senerpont Domis
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Years of Mesocosm Experiments
Description of Facility

indoor/outdoor – pelagic – freshwater
Limnotrons, outdoor enclosure facility, 36 experimental ponds

Controlled Parameters
Research Topics
Primary interests
Specialist areas
Source of Information
Photos of experiments/installations
Limnotron – a highly controlled experimental unit
Infrastructure (short name)
Limnotrons
Modality of access

Modality of access under AQUACOSM: At least 900 person days will be allocated to external users in years 2-4 of AQUACOSM Transnational Access provision. Access is offered to a minimum of 10 persons for 30 days each year.

Modality of access under AQUACOSM-plus: At least 600 person days of AQUACOSM-plus Transnational Access provision will be allocated to external users. It is anticipated that AQUACOSM-plus will support a minimum of 12 persons for 25 days per year in M10-21 and M34-45.

Unit of access
What service and support facilities are available

Services currently offered by the infrastructure: Individual external users and user groups will have access to the full range of facilities at the NIOO-KNAW. These include: fluorescence microscopes; autoanalyzer for dissolved nutrients, multichannel probes for simultaneous measurements of oxygen, light, pH, conductivity and temperature, PhytoPAM, fluorometer; Coulter particle counter, Flow cytometers for phyto- and bacterioplankton analyses, FlowCAM for analysis of large phyto- and zooplankton, Elemental analyser (particulate C and N contents), Isotope-ratio mass spectrometry (relative abundance of stable isotopes), ICP-MS (quantify trace elements), LC-MS/MS (small organic chemicals, cyanotoxins), modern molecular-biology lab. The facilities are frequently used for experiments by teams of international researchers from different disciplines including ecologists, microbiologists, biogeochemists, and evolutionary biologists. In 2019, an international multidisciplinary team working on climate change and carbon cycling is hosted. This team consist of both internal and external users, assisted by a team of highly skilled technicians at NIOO- KNAW. The laboratory’s broad range of state-of-the-art analytical equipment allows users to tackle innovative, multi- facetted questions, resulting in publications in leading journals. Users will be embedded in an internationally renowned aquatic ecology group providing ample opportunities for networking and large potential for fruitful scientific exchange. The NIOO-KNAW as a whole (~200 employees) provides an international working community, hosting several world- leading scientists.

Support offered under AQUACOSM: Users will be given full access to the facilities at the NIOO, and receive all training and assistance necessary to operate instruments and facilities before starting and during experiments. At present, the facilities are used by an international multidisciplinary team working on climate change and carbon cycling. This team consist of both internal and external users and is facilitated by a team of highly skilled technicians at NIOO. The laboratory’s broad range of state-of-the-art analytical equipment allows users to tackle innovative, multi-facetted questions, resulting in publications in leading journals. Users will be embedded in an internationally renowned aquatic ecology group providing ample opportunities for networking and large potential for fruitful scientific exchange. The NIOO as a whole (~200 employees) provides an international working community, hosting several world-leading scientists.

Support offered under AQUACOSM-plus: Users will be given full access to the facilities at NIOO-KNAW and receive training and assistance necessary to operate instruments before and during experiments.

Accommodation
Special rules