AQUACOSM-plus
Marine plankton community responses to terrestrial dissolved organic matter input and water temperature increase
A in situ mesocosm experiment to simulate a “terrestrial input extreme event”, alone, and also combined with water temperature increase will be realized in the frame of the French ANR national project entitled: Microbial responses to terrestrial dissolved organic matter input in freshwater and marine ecosystems in a changing environment (RESTORE). This marine in situ mesocosm experiment, which will be carried out in the Pilot Supersite of North-Western Mediterranean Sea, will also serve to improve synergies between Jerico-S3 (Marine coastal observatories, facilities, expertise and data for Europe) observing community and AQUACOSM-plus mesocosm experimenting community.
Graduate, Ph.D. students and young scientists (post-Doc) are very welcome for training and participating in this experiment. All trainees will be supervised by the local team.
Individuals or “Group of scientists” expert in the following topics are also welcome to contact the local scientists for propositions to study:
– Viral infection
– Plankton parasites diversity, abundance and activity
– Phytoplankton diversity and abundance,
– Ciliates, tintinnides and heterotrophic dinoflagellates diversity and abundance
– Metazooplankton diversity, abundance and activity
– Mixotrophes diversity, abundance and activity
– Detrital matter (TEP, etc.)
– Settled matter
Project lead: Dr. Behzad Mostajir and Dr. Francesca Vidussi (Behzad.Mostajir@umontpellier.fr & Francesca.Vidussi@cnrs.fr)
Timing: 19 April to 21 May 2021
Deadline for TA application at CNRS-MEDIMEER is 26 February 2021, 13:00 CET.
AQUACOSM (call is closed)
The mesocosm facility does not open for TA in 2021 in the frame of AQUACOSM.
The mesocosm facility does not open for Transnational Access in 2020.
“Summer in Spring”, Heat wave effects on Mediterranean Plankton communities: Resistance, Resilience and Recovery (Heat Plank 3R). Project Lead: Francesca Vidussi and Behzad Mostajir
Timing: Planed for one month during May-June 2019
TA-Applicants: 10 Scientists, students and trainees are strongly invited to participate
It is now increasing evidence that Climate Change affects extreme weather. In particular, the hottest days are now hotter, and the number of the so called heat waves (periods of extreme high temperature for the season) worldwide has increased. Therefore, very extreme unusual events occurring more frequently now, like “Summer in Spring” episodes (i. e. June 2017 in western Europe).
There is no doubt that climate warming impacts marine ecosystems and major changes are expected in their functioning in the near future. It is known that warming impact the structure and functioning of the plankton food web in coastal water, in particular in shallow ones that are highly sensitive to climate warming (Vidussi et al. 2011). Therefore, shallow coastal ecosystems of ecological and economic interests (i. e. exploited areas for aquaculture) are particularly vulnerable to heat waves. Moreover, in the case of the heat waves, since the resistance and resilience of the different planktonic communities facing warming could be different, the recovery of the whole plankton community after the extreme event could result in a restructuration and a consequent modification of functioning.
To study the effect of heat-waves, on coastal planktonic Mediterranean communities focusing on their resistance, resilience and recovery, a mesocosm experiment will be conducted using several pelagic in situ mesocosms of MEDIMEER. The in situ mesocosms will be immerged in the natural water column and therefore its water temperatures follow the natural water temperature variations during the day/night periods as well as during several weeks of the experimentations. To simulate a “Summer in Spring event”, the water column of several mesocosms will be increased 4-5°C to simulate a heat-waves event and maintained during 4-5 days at 4-5°C higher than the other in situ mesocosms with natural water temperature which act as control mesocosms. After these 4-5 days of heating waves, the applied increase of water temperature will be stopped and all mesocosms (heated and control) will have the same natural ambient water temperature. By applying a daily sampling during the experiment, the effects of heat-waves, including the resistance, resilience and recovery of the planktonic communities will be investigated.
This mesocosm experiment is planted to be realized during one month in May-June 2019. This experiment will be an occasion to study the plankton communities or processes that the local team does not study like plankton parasites, virioplankton or preferential nutrient assimilation by different groups of phytoplankton or bacteria, etc. It is also an occasion to test new methods to study the plankton food web components or functioning like use of underwater camera or cytometry to study large and small plankton, or other underwater instruments like in situ Fast repetition fluorometer to study photosynthesis, etc. Graduate students at different levels (Master, Ph.D., etc.) are very welcome for training and participating in this experiment, all trainees will be supervised by the local team. International scientists and students are invited to contact the local scientists for propositions (Francesca.Vidussi@umontpellier.fr & Behzad.Mostajir@umontpellier.fr).
About 10 scientists including students and trainees can participate in this experiment.
Vidussi F, Mostajir B et al. (2011). Effects of experimental warming and increased ultraviolet B radiation on the Mediterranean plankton food web. Limnology & Oceanography 56 (1): 206-218
WARM PLANK – Effects of warming on the Mediterranean plankton food web functioning. Project lead: Behzad Mostajir. 03-24/04 2018 & 02-23/10 2018
Effect of increasing water temperature simulating global warming on the Mediterranean plankton food web functioning will be investigated using the pelagic in situ mesocosms of MEDIMEER in spring and fall 2018. In each of spring and fall mesocosm experiment approved Transnational Access users can participate. The applicants are encouraged to participate to both experiments; however this is not an obligation. A total of 252 person days can be allocated to external users involved AQUACOSM Transnational Access provision in 2018. We offer access to 10-12 persons for 20-25 days in 2018. The access cost offered by MEDIMEER is based on real cost calculation, which includes all needed preparatory work (before and at site), as well as specific training needed at the sites to complete the specific projects.
The spring experiment will be realized from April 03 2018 during 3 weeks depending on meteorological conditions. This experiment is part of the PHOTOPHYTO project funded by the French National Research Agency (ANR). It focuses on the effects of global warming on the timing of marine phytoplankton blooms taking into account the different interactions between biotic and abiotic components, specially the match/mismatch between predator/prey. The in situ mesocosms are immerged in the natural water column and therefore its water temperatures follow the natural water temperature variations during the day/night periods as well as during the several weeks of the experimentations. The water column of several mesocosms will be increased and maintained at +3°C relative to other in situ mesocosms which act as CONTROLS mesocosms (Vidussi et al. 2011).
In the spring experiment, the large majority of physicochemical parameters and biological ones such as abundances, biomasses of organisms and related processes (primary production, respiration, growth, grazing, etc.) will be investigated by the scientists involved in the PHOTOPHYTO consortium. However, as a TA, international scientists are welcome to participate to this experiment for specific topics such as viral ecology, parasitism, molecular diversity, metabolomics, biochemical composition of plankton, taxonomy of phytoplankton and zooplankton, prey/predator interactions (note that the dilution experiments are already scheduled). Master and PhD students are also welcome for training and they will be supervised by the local team. In addition, by collaborating with the local team, this experiment will be an occasion to test new ecological hypothesis regarding the warming effect on the marine plankton as well as new methods to study the plankton food web components or functioning like use of underwater camera to study larger plankton, or other underwater instruments like in situ Fast repetition fluorometer to study photosynthesis, etc. International scientists are encouraged to contact the local scientists for propositions (Please login or register to view contact information.).
As there are also some phytoplankton blooms in fall in the Mediterranean coastal water, the fall experiment with more or less a similar design and protocols will permit to study the effect of increased warming on the functioning of plankton food web in the fall after the transition of the hot Mediterranean summer. The fall experiment will be carried out from October 02 2018 during 3 weeks depending on meteorological conditions.
Vidussi F, Mostajir B et al. (2011). Effects of experimental warming and increased ultraviolet B radiation on the Mediterranean plankton food web. Limnology & Oceanography 56 (1): 206-218
Sète, France
Description of the infrastructure: MEDIMEER is a unique platform on the French Mediterranean coast designed to run in-situ mesocosm experiments in coastal waters (Fig.9.14). The facility, with up to 12 pelagic mesocosms (1.2 m diameter, 2 m depth, ~2 m3 volume each), is moored in the Lagoon of Thau, 20 km southwest of Montpellier, hosted by the Marine Station of Sète, which belongs to the University of Montpellier (Dep.1). The mesocosms are attached to pontoons, maintained by a floating structure made of stainless steel and moored in the lagoon to provide natural water- temperature and light conditions. Six mesocosms can be fitted with a sensor array, enabling the acquisition of high- frequency data during experiments. Moreover, instruments for continuous measurements of air temperature and solar irradiance are installed on the same pontoon.
Several laboratories immediately adjacent to the mesocosm infrastructure are dedicated to participating in MEDIMEER experiments. They provide access to 2 wet laboratories (total area of 35 m2), 2 microbiological laboratories (total area of 40 m2), chemical laboratory (15 m2), microscopy laboratory (20 m2), 4 multitask laboratories (total of 65 m2 for sample processing), workshop (50 m2) and office space (60 m2) in the nearby buildings of the Marine Station of Sète. Available equipment and instruments include spectrofluorometer, spectrophotometer with an integrating sphere, oxygen titrator, nutrient autoanalyser, flow cytometer (FacsCalibur, Becton Dickinson), PAM fluorometer (Aquapen, PSI), epifluorescence microscope, dissecting microsopes, and various benchtop instruments (pumps, liquid nitrogen container, balances, incubators, ovens, cooled centrifuges, hoods, and more).
Pelagic, marine, outdoor, in-situ
1. Permanent floating structure: up to 12 pelagic mesocosms (1.2 m diameter, 2 m depth, ~2 m3 volume each). Fig. 1 & 2
2. Permanent floating structure: 3. Indoor structure:
3. Mobile: LAMP is composed of 9 individual structures that can be deployed individually or bonded according to experimental design. Fig. 3
CO2, pH, nutrients, plankton composition, temperature, light, salinity, turbulence, conductivity, chlorophyll a fluorescence and dissolved oxygen concentration. Some of The data are stored and transmit in real time
Plankton food web structure and functioning and their responses to global and local stressors.
Interactions between planktonic organisms and changes in structure and function of planktonic food web.
Effects of warming, increase of ultraviolet-B radiation and pH on planktonic food web.
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Modality of access under AQUACOSM-plus: A total of 500 persons days can be allocated to external users involved in one or two mesocosm experiments during M10-45 of AQUACOSM-plus Transnational Access provision. We offer access to ca 14 persons for ca 36 days each. The access cost offered by MEDIMEER is based on Actual Cost calculation, which includes needed preparatory work (before and at site), as well as specific training needed at the sites to complete the specific projects.
Services currently offered by the infrastructure: Users will get full access to the mesocosms, laboratories and equipment available in the laboratories participating in MEDIMER. The large water volume in the mesocosms permits intense and repeated sampling for several weeks and multiple users. High-frequency data from automatic sensor measurements made before and during experiments can be provided by collaborating with local teams. To date, a number of mesocosm experiments realized through nationally funded projects and Transnational Access provision during the MESOAQUA FP7 project, have already benefitted from MEDIMEER. Topics have ranged from bacteria to zooplankton responses to various aspects global environmental changes
Support offered under AQUACOSM: All users will be offered technical, logistic and scientific support before and during the experiments. This includes:
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Access to the MEDIMER mesocosms and associated materials for experimentation
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Access to temperature and solar irradiance data recorded by the weather station at the site
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Access to a range of laboratories in the Marine Station of Sète (see above)
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Standard lab equipment such as fridges, freezers, liquid N, bench-top hoods, autoclaves, distilled water
and ultra-pure water
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Technical and logistic support and supervision for using the experimental facility
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Training on specific instruments by MEDIMEER technical staff and collaboration with local staff during sample and data analysis.
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There are also a boat, aquaculture tanks, cold room, and possibility for animal collection by a diver
Support offered under AQUACOSM-plus: Users are offered access to the facility described above, including technical, logistic and scientific support. In addition, they will have access to meteorological and solar irradiance data recorded, receive training by MEDIMEER technical staff and collaboration with the local scientific team during sampling and data analysis. Boats and divers can also be provided.
Dormitories at the Marine Station of Sète or possibility to rent the hotel rooms or a villa.
