EU network of mesocosms facilities for research on marine and freshwater ecosystems open for global collaboration.

SYKE-MRC (Marine Research Centre) Mesocosm Facility

Opening for Transnational Access in 2021

There will be no opening for new TA applicants in 2021. The project planned in 2020 (see Opening for Transnational Access in 2020) was postponed to 2021 with already approved TA applicants who will be offered TA.

For new TA opportunities please go to AQUACOSM-plus.

SYKE-MRC will open their indoor facilities for Transnational Access in January-October 2020, for approximately 200 person-days. You are welcomed either to join an imaging-based planktonic food web experiment planned for August-September 2020, or to suggest your own research projects for the rest of the opening period, preferably utilizing the automated sampling and measurement system AquaBox developed at SYKE-MRC. See details below. A specific bonus for your own proposal evaluation is connection to the AQUACOSM Joint Mesocosm Experiment theme, the effect of DOM increase (‘brownification’) on food web dynamics, but all other initiatives will be carefully evaluated as well.

AQUACOSM-approved TA users are welcomed in 2020, based on negotiations with interested applicants and depending on the size of the group. In 2020, we could host 1-4 groups of 2-8 persons, for experimental periods lasting 10-30 days.

Automated sampling and measurement system AquaBox. SYKE-MRC has developed during AQUACOSM a unique automated sampling and real-time measurement system, which is modular in the sense that the flow-through system can be equipped with a wide variety of state-of-the-art instruments and analyzers at our disposal, depending on the experimental target. We have used in different AquaBox setups e.g. optical sensors for multiple phytoplankton pigments, CDOM and active fluorescence (PAM, FRRF), gas measurements (O2, pCO2), laser diffraction particle size and distribution analyser (LISST 200X), imaging instruments (FlowCytobot), and wet chemistry (FIAlab). We plan to test and develop AquaBox interfaces for several additional instruments, especially for automated imaging of planktonic compartments from bacteria to zooplankton (from flow cytometry to holographic imaging), during 2019-2020.

TA connected to testing and demonstration of new sensors applicable to flow-through measurements of planktonic systems, testing of automated imaging instruments and image recognition techniques, as well as innovative automation of sampling procedures, are thus particularly encouraged.

Imaging-based planktonic food web experiment. In August-September 2020, we plan a planktonic food web manipulation experiment, utilizing the AquaBox system and especially several parallel automated imaging techniques, to detect species-level transient responses and food web resilience to perturbations. The precise timing and experimental treatments are currently negotiated, so you are welcomed to joint the discussions by your own proposal.

Our basic mesocosm facilities include 300-liter transparent indoor units, with controllable light and temperature conditions. We host a full range of indoor experimental units from 10-1 to 102 liter scales for specific experimentation, either as separate R&D projects (cf. above), or in connection to mesocosm activities. Depending on experimental targets, mesocosms/units can be filled with cultured organisms (joint SYKE & Tvärminne phytoplankton culture collection, including hundreds of species strains) or natural waters transported from brackish or freshwater environments. The logistics need to be negotiated on a case-by-case basis.

We offer excellent analytical support for TA, especially connected to plankton dynamics, as the indoor mesocosm facility is a part of the well-equipped SYKE-MRC laboratory. This support can be negotiated to contain a variety of chemical, biological and bio-optical analyses, with advanced imaging and sample preparation possibilities. The applicants are strongly encouraged to contact us prior to submitting their proposals, to negotiate most fruitful possibilities.

Contact persons:

Res. Prof. Timo Tamminen (food web dynamics, AquaBox R&D) –timo.tamminen@environment.fi

Res. Prof. Maiju Lehtiniemi (food web dynamics, zooplankton, microplastics) – maiju.lehtiniemi@ymparisto.fi

Head of SYKE-MRC laboratory Jukka Seppälä (Imaging technologies, bio-optics, AquaBox R&D) – jukka.seppala@environment.fi

Comparing the effects of conventional and biodegradable microplastics on the lower planktonic food web of the northern Baltic Sea (COMB). Project Lead: Maiju Lehtiniemi

An indoor mesocosm experiment will be carried out in 26.8. – 6.9.2019 at the facilities of Finnish Environment Institute (SYKE) in Helsinki. A pre-screened plankton community from the northern Baltic Sea will be exposed to two types of small microplastics, which are expected to have differing effects on the production and fecundity of the lower food web organisms from bacteria to zooplankton. Automated sampling and measurements of the changes in the food web structure and functioning will be conducted during the experiment. Ingestion of microplastics will be examined with epifluoresence microscopy from pre-stained samples.  Additional side experiments are performed to estimate the fecundity of zooplankton under the different manipulation environments.

We encourage a wide range of Transnational Access (TA) users to apply: You may come from public authorities, technological partners, and research or teaching institutions. As we plan to observe whole-ecosystem responses, everyone with expertise or interest in plankton communities or pelagic processes is welcome to apply. We specifically invite researchers and trainees with expertise or interest towards mesozooplankton fecundity (e.g. egg production) to apply. We will also deploy video-based in situ zooplankton recorders, for this task persons with interest in zooplankton and/or programming are welcome to apply.

A total of up to 250 person days will be allocated to external users through Transnational Access provided under AQUACOSM for 2019. It is anticipated that AQUACOSM will support stays of at least 5 persons for ca. 50 days, or potentially other combinations.

If you are interested, please contact Maiju.Lehtiniemi@ymparisto.fi immediately to get advice about possible options.

SYKE will open their indoor facilities for August-December 2018 for two areas of interest to potential participants, both mainly aimed to facilitate methodological developments relevant for the AQUACOSM community and a broader network:   

1) piloting automated measurement systems applicable to mesocosm experimentation on planktonic communities, especially novel imaging and bio-optical techniques (i.e. encouraging analyzer providers and researchers to demonstrate their equipment in a setup with a wide array of reference measurements on plankton dynamics)

2) testing the (side) effects of artificial DOC additions on variable optical measurements

Other initiatives will be carefully evaluated as well. AQUACOSM approved TA users are welcomed for August-December 2018, based on negotiations with interested applicants and depending on the size of the group. SYKE-MRC laboratories have been moved into new premises. We have on average 110 person-days TA allocated per year (for three years). In 2018 we could host 1-3 groups of 2-4 persons, for experimental periods lasting 10-30 days (totaling  max 110 person-days).

The main effort of SYKE -MRC indoor facility in 2018 will focus on R&D on automated sampling and measurement systems to be developed in AQUACOSM WP8. TA connected to testing and demonstration of new sensors applicable to flow-through measurements of planktonic systems, as well as innovative automation of sampling procedures, are thus particularly encouraged.

Our basic mesocosm facilities include 300-liter transparent indoor units, with controllable light and temperature conditions. We host a full range of indoor experimental units from 10-1 to 102 liter scales for specific experimentation, either as separate R&D projects (cf. above), or in connection to mesocosm activities. Depending on experimental targets, mesocosms/units can be filled with cultured organisms (joint SYKE & Tvärminne phytoplankton culture collection, including hundreds of species strains) or natural waters transported from brackish or freshwater environments. These logistics need to be negotiated on a case-by-case basis.

We offer excellent analytical support for TA, especially connected to plankton dynamics, as the indoor mesocosm facility is a part of the SYKE-MRC laboratory. This support can be negotiated to contain a variety of chemical, biological and bio-optical analyses, with advanced imaging and sample preparation possibilities. The applicants are strongly encouraged to contact us to negotiate most fruitful possibilities prior to submitting their proposals.

Legal name of organisation (short name)
Marine Research Centre
Country
Finland
Continent
Europe
Organisation address

Marine Research Centre (MRC)
Finnish Environment Institute SYKE
Latokartanonkaari 11, 00790 Helsinki FINLAND

Infrastructure (short name)
SYKE-MRC (Marine Research Centre) Mesocosm Facility
Infrastructure address

Helsinki, Finland

Coordinates / (routes, areas if non-static)
Facility location(s)
Description of the Infrastructure

Description of the infrastructure: The SYKE-MRC Indoor Mesocosm Facility is part of SYKE’s Marine Ecology Laboratory hosting indoor experimental systems in a range of volumes up to 300 L. Temperature and light conditions are well controlled. The facility has several walk-in climate chambers and modern laboratories equipped with a wide range of instruments, including advanced bio-optical methods, such as flow cytometer, Cytosense, imaging FlowCam, high- quality microscopes, spectrophotometer with integrating sphere, spectrofluorometers, FRR fluorometers, plate readers, controllable LED panels, nutrient analysers, and an isotope laboratory. Furthermore, SYKE-MRC Indoor Mesocosm is associated with a phytoplankton culture collection including hundreds of strains that can be used in experimental studies.

SYKE-MRC Indoor Mesocosm Facility hosts a wide range of temperature and light-controlled experimental units up to 300 L (left), attractive to teams of international experts (right).
Primary contact information (PI)

Res. Prof. Timo Tamminen
Head of Laboratory Jukka Seppälä

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Years of Mesocosm Experiments
periodically since 1988
Description of Facility

pelagic – marine – indoor

Indoor experimental systems in a range of volumes, with integrated incubation condition control, data retrieval, and their on-line presentation. Also ice tanks available for ice ecology research

 

Controlled Parameters

Temperature, light, pH, pCO2, nutrient levels, community composition

Research Topics

Planktonic food web, phytoplankton ecology, stoichiometry, biodiversity, ice ecology

Primary interests
Specialist areas
Photos of experiments/installations
ndoor medium-scale experimental unit
SYKE-MRC field mesocosm work at Tvärminne

 
Infrastructure (short name)
SYKE-MRC (Marine Research Centre) Mesocosm Facility
Modality of access

Modality of access under AQUACOSM:total of at least 336 person-days will be allocated to external users through AQUACOSM Transnational Access provision in years 2-4. Access is offered to a minimum of 4 persons for an average of 20 days each year. Access costs will be declared on the basis of a combination of Unit and Actual Costs (186 € and 165 € per user-day, respectively), the latter covering specific support from SYKE-MRC staff.

Modality of access under AQUACOSM-plus: A total of at least 240 person-days will be allocated to external users through AQUACOSM-plus TA provision. Access is offered to a minimum of 6 persons for an average of 20 days per year within M10-45. However, a smaller user numbers per year is preferred to distribute over 3 years. Access costs will be declared on the basis of a combination of Unit and Actual Costs, the latter covering specific support from SYKE- MRC staff.

Unit of access
What service and support facilities are available

Services currently offered by the infrastructure: External users have full access to the SYKE-MRC Mesocosm Facility and benefit from additional support provided by the associated facilities. Technical and analytical support by experienced staff is available when costs are covered. Access to the basic facility includes preparation of mesocosms for experiments or other uses, including sampling and sample transport of natural water or organisms, basic physico-chemical and bio-optical monitoring of the experimental units with the automated sampling and analysis platform AquaBox, equipment for sampling and sample preparation, wet-lab and laboratory bench space needed by users bringing their own instrumentation. Comparisons, calibration and validation of bio-optical instruments, and tests of real-time data acquisition systems, representing the specific expertise of the scientific staff, is offered to external users. In addition to ecological experiments on plankton, the indoor mesocosm facility provides an attractive platform for methodological R&D investigations by external users e.g. topics related Blue Growth or instrument development and testing under variable but controlled environmental and biological conditions.

Support offered under AQUACOSM: Users under AQUACOSM have access to the indoor mesocosms, lab space, instruments and service by scientific, technical and administrative staff. to lab space and the associated facilities, to standard disposables and administrative and logistic support and to laboratory services listed above, as agreed upon in a user-access contract. Staff will provide training in the use of the facilities where necessary. Advanced technical or analytical support by SYKE-MRC researchers and laboratory technicians is available upon request within the limits set by available Actual Costs.

Support offered under AQUACOSM-plus: Users have access to the indoor mesocosms, lab space, instruments and service by scientific, technical and administrative staff, the associated facilities, standard disposables and administrative and logistic support and laboratory services listed above, as agreed upon in a user-access contract. Staff will provide training in the use of the facilities where necessary. Advanced technical or analytical support by SYKE-MRC researchers and laboratory technicians is available upon request within the limits set by available Actual Costs.

Accommodation

City location – commercial lodging

Special rules